Chapter 26
The Urinary System

Kidneys, ureters, urinary bladder & urethra

Urine flows from each kidney, down its ureter to the bladder and to the outside via the urethra

Filter the blood and return most of water and solutes to the bloodstream

Overview of Kidney Functions

Regulation of blood ionic composition

Na+, K+, Ca+2, Cl- and phosphate ions

Regulation of blood pH, osmolarity & glucose

Regulation of blood volume

conserving or eliminating water

Regulation of blood pressure

secreting the enzyme renin

adjusting renal resistance

Release of erythropoietin & calcitriol

Excretion of wastes & foreign substances

 

External Anatomy of Kidney

Paired kidney-bean-shaped organ

4-5 in long, 2-3 in wide,
1 in thick

Found just above the waist between the peritoneum & posterior wall of abdomen

retroperitoneal along with adrenal glands & ureters

Protected by 11th & 12th ribs with right kidney lower

 

External Anatomy of Kidney

Blood vessels & ureter enter hilus of kidney

Renal capsule = transparent membrane maintains organ shape

Adipose capsule that helps protect from trauma

Renal fascia = dense, irregular connective tissue that holds against back body wall

Internal Anatomy of the Kidneys

Parenchyma of kidney

renal cortex = superficial layer of kidney

renal medulla

inner portion consisting of 8-18 cone-shaped renal pyramids separated by renal columns

renal papilla point toward center of kidney

Drainage system fills renal sinus cavity

cuplike structure (minor calyces) collect urine from the papillary ducts of the papilla

minor & major calyces empty into the renal pelvis which empties into the ureter

Internal Anatomy of Kidney

What is the difference between renal hilus & renal sinus?

Outline a major calyx & the border between cortex & medulla.

Blood & Nerve Supply of Kidney

Abundantly supplied with blood vessels

receive 25% of resting cardiac output via renal arteries

Functions of different capillary beds

glomerular capillaries where filtration of blood occurs

vasoconstriction & vasodilation of afferent & efferent arterioles produce large changes in renal filtration

peritubular capillaries that carry away reabsorbed substances from filtrate

Sympathetic vasomotor nerves regulate blood flow & renal resistance by altering arterioles

 

 

Blood Vessels around the Nephron

Glomerular capillaries are formed between the afferent & efferent arterioles

Efferent arterioles give rise to the peritubular capillaries and vasa recta

Blood Supply to the Nephron

The Nephron

Kidney has over 1 million nephrons composed of a corpuscle and tubule

Renal corpuscle = site of plasma filtration

glomerulus is capillaries where filtration occurs

glomerular (Bowman’s) capsule is double-walled epithelial cup that collects filtrate

Renal tubule

proximal convoluted tubule

loop of Henle dips down into medulla

distal convoluted tubule

Collecting ducts and papillary ducts drain urine to the renal pelvis and ureter

 

Cortical Nephron

80-85% of nephrons are cortical nephrons

Renal corpuscles are in outer cortex and loops of Henle lie mainly in cortex

Juxtamedullary Nephron

15-20% of nephrons are juxtamedullary nephrons

Renal corpuscles close to medulla and long loops of Henle extend into deepest medulla enabling excretion of dilute or concentrated urine

Histology of the Nephron & Collecting Duct

Single layer of epithelial cells forms walls of entire tube

Distinctive features due to function of each region

microvilli

cuboidal versus simple

hormone receptors

Structure of Renal Corpuscle

Bowman’s capsule surrounds capsular space

podocytes cover capillaries to form visceral layer

simple squamous cells form parietal layer of capsule

Glomerular capillaries arise from afferent arteriole & form a ball before emptying into efferent arteriole

 

Juxtaglomerular Apparatus

Structure where afferent arteriole makes contact with ascending limb of loop of Henle

macula densa is thickened part of ascending limb

juxtaglomerular cells are modified muscle cells in arteriole

Number of Nephrons

Remains constant from birth

any increase in size of kidney is size increase of individual nephrons

If injured, no replacement occurs

Dysfunction is not evident until function declines by 25% of normal (other nephrons handle the extra work)

Removal of one kidney causes enlargement of the remaining until it can filter at 80% of normal rate of 2 kidneys

Overview of Renal Physiology

Nephrons and collecting ducts perform 3 basic processes

glomerular filtration

a portion of the blood plasma is filtered into the kidney

tubular reabsorption

water & useful substances are reabsorbed into the blood

tubular secretion

wastes are removed from the blood & secreted into urine

Rate of excretion of any substance is its rate of filtration, plus its rate of secretion, minus its rate of reabsorption

Overview of Renal Physiology

Glomerular filtration of plasma

Tubular reabsorption

Tubular secretion

Glomerular Filtration

Blood pressure produces glomerular filtrate

Filtration fraction is 20% of plasma

48 Gallons/day
filtrate reabsorbed
to 1-2 qt. urine

Filtering capacity
enhanced by:

thinness of membrane & large surface area of glomerular capillaries

glomerular capillary BP is high due to small size of efferent arteriole

Filtration Membrane

#1 Stops all cells and platelets

#2 Stops large plasma proteins

#3 Stops medium-sized proteins, not small ones

Glomerular Filtration Rate

Amount of filtrate formed in all renal corpuscles of both kidneys / minute

average adult male rate is 125 mL/min

Homeostasis requires GFR that is constant

too high & useful substances are lost due to the speed of fluid passage through nephron

too low and sufficient waste products may not be removed from the body

Changes in net filtration pressure affects GFR

filtration stops if GBHP drops to 45mm Hg

functions normally with mean arterial pressures 80-180

Renal Autoregulation of GFR

Mechanisms that maintain a constant GFR despite changes in arterial BP

myogenic mechanism

systemic increases in BP, stretch the afferent arteriole

smooth muscle contraction reduces the diameter of the arteriole returning the GFR to its previous level in seconds

tubuloglomerular feedback

elevated systemic BP raises the GFR so that fluid flows too rapidly through the renal tubule & Na+, Cl- and water are not reabsorbed

macula densa detects that difference & releases a vasoconstrictor from the juxtaglomerular apparatus

afferent arterioles constrict & reduce GFR

Neural Regulation of GFR

Blood vessels of the kidney are supplied by sympathetic fibers that cause vasoconstriction of afferent arterioles

At rest, renal BV are maximally dilated because sympathetic activity is minimal

renal autoregulation prevails

With moderate sympathetic stimulation, both afferent & efferent arterioles constrict equally

decreasing GFR equally

With extreme sympathetic stimulation (exercise or hemorrhage), vasoconstriction of afferent arterioles reduces GFR

lowers urine output & permits blood flow to other tissues

Tubular Reabsorption & Secretion

Normal GFR is so high that volume of filtrate in capsular space in half an hour is greater than the total plasma volume

Nephron must reabsorb 99% of the filtrate

PCT with their microvilli do most of work with rest of nephron doing just the fine-tuning

solutes reabsorbed by active & passive processes

water follows by osmosis

small proteins by pinocytosis

Important function of nephron is tubular secretion

transfer of materials from blood into tubular fluid

helps control blood pH because of secretion of H+

helps eliminate certain substances (NH4+, creatinine, K+)

 

Transport Mechanisms

Water is only reabsorbed by osmosis

obligatory water reabsorption occurs when water is “obliged” to follow the solutes being reabsorbed

facultative water reabsorption occurs in collecting duct under the control of antidiuretic hormone

 

Glucosuria

Common cause is diabetes mellitis because insulin activity is deficient and blood sugar is too high

 

 

Reabsorption in the Loop of Henle

Tubular fluid

PCT reabsorbed 65% of the filtered water so chemical composition of tubular fluid in the loop of Henle is quite different from plasma

since many nutrients were reabsorbed as well, osmolarity of tubular fluid is close to that of blood

Sets the stage for independent regulation of both volume & osmolarity of body fluids

 

Symporters in the Loop of Henle

Thick limb of loop of Henle has Na+ K- Cl- symporters that reabsorb these ions

K+ leaks through K+ channels back into the tubular fluid leaving the interstitial fluid and blood with a negative charge

Cations passively move to the vasa recta

Reabsorption & Secretion in the Collecting Duct

By end of DCT, 95% of solutes & water have been reabsorbed and returned to the bloodstream

Cells in the collecting duct make the final adjustments

principal cells reabsorb Na+ and secrete K+

intercalated cells reabsorb K+ & bicarbonate ions and secrete H+

Actions of the Principal Cells

Na+ enters principal cells
through leakage channels

Na+ pumps keep the
concentration of Na+ in
the cytosol low

Cells secrete variable
amounts of K+, to adjust
for dietary changes in K+
intake

down concentration gradient due to Na+/K+ pump

Aldosterone increases Na+ and water reabsorption & K+ secretion by principal cells by stimulating the synthesis of new pumps and channels.

Secretion of H+ and Absorption of Bicarbonate by Intercalated Cells

Proton pumps (H+ATPases) secrete H+ into tubular fluid

can secrete against a concentration gradient so urine can be 1000 times more acidic than blood

 

Hormonal Regulation

Hormones that affect Na+, Cl- & water reabsorption and K+ secretion in the tubules

angiotensin II and aldosterone

decreases GFR by vasoconstricting afferent arteriole

enhances absorption of Na+

promotes aldosterone production which causes principal cells to reabsorb more Na+ and Cl- and less water

increases blood volume by increasing water reabsorption

 

Antidiuretic Hormone

Increases water permeability of principal cells so regulates facultative water reabsorption

When osmolarity of plasma & interstitial fluid decreases, more ADH is secreted and facultative water reabsorption increases.

Production of Dilute or Concentrated Urine

Homeostasis of body fluids despite variable fluid intake

Kidneys regulate water loss in urine

ADH controls whether dilute or concentrated urine is formed

if lacking, urine contains high ratio of water to solutes

Formation of Dilute Urine

Dilute = having fewer solutes than plasma (300 mOsm/liter).

diabetes insipidus

Filtrate and blood have equal osmolarity in PCT

Principal cells do not reabsorb water if ADH  is low

Formation of Concentrated Urine

Compensation for low water intake or heavy perspiration

Urine can be up to 4 times greater osmolarity than plasma

Cells in the collecting ducts reabsorb more water & urea when ADH is increased

 

Summary

H2O Reabsorption

PCT---65%

loop---15%

DCT----10-15%

collecting duct---       5-10% with ADH

Dilute urine has not had enough water removed, although sufficient ions have been reabsorbed.

Reabsorption within Loop of Henle

Diuretics

Substances that slow renal reabsorption of water & cause diuresis (increased urine flow rate)

caffeine which inhibits Na+ reabsorption

alcohol which inhibits secretion of ADH

prescription medicines can act on the PCT, loop of Henle or DCT

Evaluation of Kidney Function

Urinalysis

analysis of the volume and properties of urine

normal urine is protein free, but includes filtered & secreted electrolytes

urea, creatinine, uric acid, urobilinogen, fatty acids, enzymes & hormones

Blood tests

blood urea nitrogen test (BUN) measures urea in blood

rises steeply if GFR decreases severely

plasma creatinine--from skeletal muscle breakdown

renal plasma clearance of substance from the blood in ml/minute (important in drug dosages)

Dialysis Therapy

Kidney function is so impaired the blood must be cleansed artificially

separation of large solutes from smaller ones by a selectively permeable membrane

Artificial kidney machine performs hemodialysis

directly filters blood because blood flows through tubing surrounded by dialysis solution

cleansed blood flows back into the body

Anatomy of Ureters

10 to 12 in long

Varies in diameter from 1-10 mm

Extends from renal pelvis to bladder

Retroperitoneal

Enters posterior wall of bladder

Physiological valve only

bladder wall compresses arterial opening as it expands during filling

flow results from peristalsis, gravity & hydrostatic pressure

Histology of Ureters

3 layers in wall

mucosa is transitional epithelium & lamina propria

since organ must inflate & deflate

mucus prevents the cells from being contacted by urine

muscularis

inner longitudinal & outer circular smooth muscle layer

distal 1/3 has additional longitudinal layer

peristalsis contributes to urine flow

 

Location of Urinary Bladder

Posterior to pubic symphysis

In females is anterior to vagina & inferior to uterus

In males lies anterior to rectum

Anatomy of Urinary Bladder

Hollow, distensible muscular organ with capacity of 700 - 800 mL

Trigone is smooth flat area bordered by 2 ureteral openings and one urethral opening

Histology of Urinary Bladder

3 layers in wall

mucosa is transitional epithelium & lamina propria

since organ must inflate & deflate

mucus prevents the cells from being contacted by urine

muscularis (known as detrusor muscle)

3 layers of smooth muscle

inner longitudinal, middle circular & outer longitudinal

circular smooth muscle fibers form internal urethral sphincter

circular skeletal muscle forms external urethral sphincter

adventitia layer of loose connective tissue anchors in place

superior surface has serosal layer (visceral peritoneum)

Micturition Reflex

Micturition or urination (voiding)

Stretch receptors signal spinal cord and brain

when volume exceeds 200-400 mL

Impulses sent to micturition center in sacral spinal cord (S2 and S3) & reflex is triggered

parasympathetic fibers cause detrusor muscle to contract, external & internal sphincter muscles to relax

Filling causes a sensation of fullness that initiates a desire to urinate before the reflex actually occurs

conscious control of external sphincter

cerebral cortex can initiate micturition or delay its occurrence for a limited period of time

Anatomy of the Urethra

Females

length of 1.5 in., orifice between clitoris & vagina

histology

transitional changing to nonkeratinized stratified squamous epithelium, lamina propria with elastic fibers & circular smooth muscle

Males

tube passes through prostate, UG diaphragm & penis

3 regions of urethra

prostatic urethra, membranous urethra & spongy urethra

circular smooth muscle forms internal urethral sphincter & UG diaphragm forms external urethral sphincter

 

Urinary Incontinence

Lack of voluntary control over micturition

normal in 2 or 3 year olds because neurons to sphincter muscle is not developed

Stress incontinence in adults

caused by increases in abdominal pressure that result in leaking of urine from the bladder

coughing, sneezing, laughing, exercising, walking

injury to the nerves, loss of bladder flexibility, or damage to the sphincter

Aging and the Urinary System

Anatomical changes

kidneys shrink in size from 260 g to 200 g

Functional changes

lowered blood flow & filter less blood (50%)

diminished sensation of thirst increases susceptibility to dehydration

Diseases common with age

acute and chronic inflammations & canaliculi     

infections, nocturia, polyuria, dysuria, retention or incontinence and hematuria

Cancer of prostate is common in elderly men

Disorders of Urinary System

Renal calculi

Urinary tract infections

Glomerular disease

Renal failure

Polycystic kidney disease